RDML-RunAnalysis

Main
RunView
AmplificationCurveAnalysis
MeltingCurveAnalysis
Corrected Data
Help

Load RDML file

RDML file (.rdml, .rdm or .xml)

Excluding samples with diverging individual PCR efficiency:

Update RDML Data:

Exclude from mean efficiency calculation:

Samples without plateau phase:

PCR median efficiency range:

Exclude from calculations:

Samples with unstable baseline:

Update Target PCR Efficiency:

Table Format:

Decimal Separator:

Include Annotations:

Normalisation:

Fluor Loss:

True Peak Tolerance:

°C

Update RDML Data:

Artifact Peak Tolerance:

°C

Min Peak Temp:

°C

Max Peak Temp:

°C

Max Peak Width:

°C

Normalisation Parameters:

Exponential:

Low:

°C

High:

°C

Bilinear:

Low:

°C -

°C

High:

°C -

°C

Ignore Peaks With Size Below:

Format:

Decimal Separator:

Application Description

RDML-RunAnalysis is a tool to view single and analyze a single run of a RDML files. Once the RDML file was loaded, the desired experiment and run have to be selected. RDML-RunAnalysis will display the plate setup and the amplification- or meltcurves. The “AmplificationCurveAnalysis” tab allows to analyze amplification curves and calculate TD0, PCR efficiency and Ncopy. The "MeltingCuveAnalysis" tab calculates the melting temperatures of the products.

A detailed introduction on analyzing runs is available at RDML-Tools Help - Analyze a Run with RDML-RunAnalysis.

Accepted Input

The RDML files can be provided in rdml or xml format (*.rdml, *.rdm, and *.xml).

Sample Data

Version

rdmlpython version: updated on server interaction

More Help

A general help file is available as RDML-Tools Help.